The sulphation of chondroitin sulphate in embryonic chicken cartilage
نویسندگان
چکیده
منابع مشابه
Age-related changes in the sulphation of the chondroitin sulphate linkage region from human articular cartilage aggrecan.
The chondroitin sulphate (CS) linkage regions have been isolated from human articular cartilage aggrecan (from 10- to 72-year-olds) by chondroitin ABC endolyase digestion and size-exclusion chromatography. Linkage region hexasaccharides have been characterized and their abundance estimated by high-pH anion-exchange chromatography. The basic structure for the CS linkage region oligosaccharides i...
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Chondroitin sulphate chains on cell and extracellular matrix proteoglycans play important regulatory roles in developing systems. Specific, developmentally regulated, sulphation motifs within the chondroitin glycosaminoglycan structure may help bind, sequester or present bioactive signalling molecules to cells thus modulating their behaviour. Using monoclonal antibodies 3B3(-), 4C3, 6C3 and 7D4...
متن کاملImmunochemical and biochemical comparisons between embryonic chick bone marrow and epiphyseal cartilage chondroitin/dermatan sulphate proteoglycans.
Chrondroitin sulphate proteoglycans obtained from embryonic chick bone marrow and epiphyseal cartilage were compared using immunochemical and biochemical analyses. Proteoglycans from each tissue, separated on CsCl density gradients, under dissociative conditions, into high (1.6 g ml-1), medium (1.5 g ml-1) and low (1.4 g ml-1) buoyant density fractions, were immunochemically analysed, using a p...
متن کاملComparative biochemistry of chondroitin sulphate-proteins of cartilage and notochord.
Fragments that consisted mainly of two polysaccharide chains joined by a short polypeptide bridge (doublets) were prepared from chondroitin sulphate-proteins of lamprey, sturgeon, elasmobranch and ox connective tissues after hydrolysis with trypsin and chymotrypsin. Consideration of molecular parameters, compositions and behaviour on gel electrophoresis and density-gradient fractionation leads ...
متن کاملThe sulfation of chondroitin sulfate in embryonic chick cartilage epiphyses.
With the use of purified chondroitinase of Proteus vulgaris, a method has been devised for the determination of relative amounts of chondroitin, chondroitin-4-SO*, and chondroitin6-SO4 in cartilage. This method was applied to the analysis of embryonic chick epiphyseal cartilage. With 35S04 and ‘*C-acetate it was possible to study the relationship of embryo age to rate of formation of chondroiti...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1969
ISSN: 0306-3283
DOI: 10.1042/bj1130543